Abstract
Several addiction susceptibility genes have been mapped by linkage and genomewide association. However, functional alleles associated with disease risk have not been identified, with a few possible exceptions. In addition, little is known about the cis- and trans-acting factors involved in regulating their expression. To address these issues, we used a ChIP-chip approach to identify regulatory elements in fetal-brain- targeting genes implicated in addiction and other neuropsychiatric conditions. Our data point to a number of putative regulatory elements, several of which, we show, are functionally significant. Many established or putative regulatory elements map near-disease-associated SNPs. These regions would be of interest to survey for patient-specific functional variants involved in disease susceptibility.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Brain / metabolism
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Brain Chemistry / genetics
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Calcium-Binding Proteins
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Cell Adhesion Molecules, Neuronal / genetics*
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Cell Line, Tumor
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DNA Mutational Analysis / methods
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Fetus / metabolism
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Gene Expression Profiling / methods*
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Gene Expression Regulation / genetics
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Genetic Markers / genetics
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Genetic Testing / methods
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Humans
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Nerve Tissue Proteins / genetics*
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Neural Cell Adhesion Molecules
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Neurocognitive Disorders / genetics*
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Oligonucleotide Array Sequence Analysis / methods*
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Polymorphism, Single Nucleotide / genetics
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Protein Structure, Tertiary / genetics
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Substance-Related Disorders / genetics*
Substances
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Calcium-Binding Proteins
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Cell Adhesion Molecules, Neuronal
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Genetic Markers
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NRXN1 protein, human
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Nerve Tissue Proteins
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Neural Cell Adhesion Molecules