Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Lett. 2009 Dec 1;34(23):3668-70. doi: 10.1364/OL.34.003668.

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells.

Author information

  • 1George R. Harrison Spectroscopy Laboratory, MIT, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.

PMID:
19953156
[PubMed - indexed for MEDLINE]
PMCID:
PMC2848941
Free PMC Article

Images from this publication.See all images (3)Free text

Fig. 1
Fig. 2
Fig. 3
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America Icon for PubMed Central
    Loading ...
    Write to the Help Desk