(A, B) Representative confocal images of the BrdU-positive cells in the SGZ of female apoE3-KI (A) and apoE4-KI (B) mice at 6–7 months of age were collected 1 day after BrdU injection.
(C, D) Representative confocal images of the BrdU and NeuN double positive cells in the SGZ of female apoE3-KI (C) and apoE4-KI (D) mice at 6–7 months of age were collected 4 weeks after BrdU injection.
(E, F) Representative confocal images of the BrdU and S100β double positive cells in the SGZ of female wildtype (E) and apoE-KO (F) mice at 6–7 months of age were collected 4 weeks after BrdU injection.
(G–J) Numbers of newborn cells (BrdU⁺) (G), immature neurons (BrdU⁺/Dcx⁺) (H), mature neurons (BrdU⁺/NeuN⁺) (I), and astrocytes (BrdU⁺/S100β⁺) (J) in the SGZ of female mice of various apoE genotypes at 6–7 months of age were determined 1 and 3 days and 4 and 10 weeks after BrdU injection. Values are mean ± SD (n = 4–6 mice per genotype). * p < 0.05 vs. other groups (t test).M
(K) Total numbers of Sox2-positive cells in the SGZ of female wildtype, apoE3-KI, apoE4-KI, and apoE-KO mice at 6–7 months of age. Values are mean ± SD (n = 4 mice per genotype).M
(L) Numbers of BrdU and Sox2 double-positive cells in the SGZ of female wildtype, apoE3-KI, apoE4-KI, and apoE-KO mice at 6–7 months of age were determined 1 day after BrdU injection. Values are mean ± SD (n = 4 mice per genotype).
(M) Total numbers of Ki67-positive cells in the SGZ of female wildtype, apoE3-KI, apoE4-KI, and apoE-KO mice at 6–7 months of age. Values are mean ± SD (n = 4 mice per genotype).
(N–P) Numbers of immature neurons (BrdU⁺/Dcx⁺) (N), astrocytes (BrdU⁺/S100β⁺) (O), and mature neurons (BrdU⁺/NeuN⁺) (P) in the SGZ of female mice of various apoE genotypes at 6–7 months of age were determined at 3 days and 4 weeks after BrdU injection. Values are mean ± SD (n = 4–6 mice per genotype). * p < 0.05 vs. wildtype and apoE3-KI mice (t test).

(A–D) Immunostaining of GAD67-positive GABAergic interneurons in the hilus of female wildtype (A), apoE-KO (B), apoE3-KI (C), and apoE4-KI (D) mice at 6–7 months of age.
(E) Numbers of GAD67-positive GABAergic interneurons in different mice at 6–7 months of age. Values are mean ± SD (n = 4–7 mice per genotype). * p < 0.05 vs. wildtype and apoE3-KI mice (t test).
(F, G) Positive correlation between the number of GAD67-positive interneurons and the number of BrdU⁺/NeuN⁺ neurons among female wildtype, apoE3-KI, and apoE4-KI mice at 6–7 months of age (F, n = 12 mice) and at 3 months of age (G, n = 15 mice).
(H) GABA release in hippocampal slices, determined by mass spectrometry. Values are mean ± SD (n = 4–7 mice per genotype). * p < 0.05 vs. wildtype and apoE3-KI mice (t test).

(A, B) Sample traces of mSSCs in a GFP⁺ neuron 2 weeks after retrovirus-GFP injection from an apoE3-KI (A) or an apoE4-KI (B) mouse during whole-cell voltage clamp recording in the presence of DNQX (20 μM), D-AP5 (50 μM), and TTX (1 μM). The mSSCs were blocked by bath application of BMI (100 μM). Scale bars, 10 pA and 5 s.
(C, D) Sample traces of ePSCs in a GFP⁺ neuron at 2 weeks after retrovirus-GFP injection from an apoE3-KI (C) or an apoE4-KI (D) mouse during whole-cell voltage clamp recording in the presence of DNQX (20 μM) and D-AP5 (50 μM). Currents were blocked by bath application of BMI (100 μM). Scale bars: 10 pA and 50 ms.
(E) Average mSSC frequency in GFP⁺ neurons was lower in apoE4-KI mice than apoE3-KI mice. Values are mean ± SD (n = 21–28 cells per genotype). * p < 0.05 vs. apoE3-KI mice (t test).
(F) Average ePSC amplitude in GFP⁺ neurons was lower in apoE4-KI mice than in apoE3-KI mice. Values are mean ± SD (n = 21–28 cells per genotype). * p < 0.05 vs. apoE3-KI mice (t test).
(G) Average membrane resistance of GFP⁺ neurons in apoE3-KI and apoE4-KI mice 2 weeks after retrovirus-GFP injection. Values are mean ± SD (n = 40 cells per genotype). * p < 0.005 vs. apoE3-KI mice (t test).

(A–J)Confocal images of the dentate gyrus of EGFP_apoE reporter mice. Green indicates EGFP representing apoE (A, B, D, E, G–J). Red indicates immunostaining positive for anti-GFAP (A), anti-nestin (C, D), anti-Sox2 (F, G), anti-apoE (H), anti-Dcx (I), or anti-β-III-tubulin (J).

(A–G) Confocal three-dimensional reconstruction of dendrites (inverted images) of newborn neurons (4 weeks after retrovirus-GFP injection) in the dentate gyrus of wildtype (A), apoE3-KI (B), apoE4-KI (C), and apoE-KO (D) mice, wildtype mice treated with PB (E), apoE3-KI mice treated with PB (F), and apoE4-KI mice treated with PB (G). Scale bar, 50 µm.
(H–K) Total dendritic length (H, J) and dendritic branch number (I, K) of newborn neurons. * p < 0.05 (t test in H and I; Kolmogorov-Smirnov test in J and K). WT, n = 43; E3-KI, n = 84; E4-KI, n = 73; E-KO, n =35; WT + PB, n =52; E3-KI + PB, n =42; E4-KI + PB, n = 31. Values in panels H and I are mean ± SEM.

(A–H) Primary hippocampal neuron cultures were prepared from P0 pups of apoE3-KI, apoE4-KI, wildtype, and apoE-KO mice, cultured for 14 days in vitro (14 DIV), and stained with anti-MAP2 (red) and DAPI (blue) (A–D) or anti-GAD67 (green) and DAPI (blue) (E–H). Shown are representative images from five coverslips of each genotype and five fields per coverslip (magnification, 200x).
(I, J) MAP2-positive (I) and GAD67-positive (J) neurons were quantified. Values are mean ± SEM (five images per coverslip and five coverslips per genotype). * p < 0.05 versus other groups (t test).
(K) Anti-apoE western blot of primary neuron lysates from apoE3-KI, apoE4-KI, wildtype, and apoE-KO mice. Note that mouse apoE is 5 amino acids shorter than human apoE.
(L) ApoE fragmentation, reported as the ratio of total apoE fragments to total tau. Values are mean ± SD (n = 3–4 mice per genotype). * p < 0.001 versus other groups (t test).
(M, N) Anti-p-tau (M, AT8 monoclonal antibody) and anti-total tau (N, tau-5 monoclonal antibody) western blots of primary neuron lysates from apoE3-KI, apoE4-KI, wildtype, and apoE-KO mice.
(O) The level of tau phosphorylation, reported as the ratio of p-tau to total tau. Values are mean ± SD (n = 3–4 mice per genotype). * p < 0.001 versus other groups (t test).

Female apoE3-KI and apoE4-KI mice at 6–7 months of age were treated with a GABA_A receptor potentiator (PB, 50 mg/kg) or antagonist (PTX, 4 mg/kg) as described in the text. BrdU-positive cells in the SGZ were counted at 1 day (A) and 4 weeks (B), and immature neurons (C) and mature neurons (D) were counted at 4 weeks. Untreated wildtype mice at 6–7 months of age served as controls. Values are mean ± SD (n = 4–6 mice per genotype). * p < 0.01 vs. untreated mice of the same apoE genotype (t test).
(E) A working model for the roles of apoE and its isoforms in adult hippocampal neurogenesis. Adult hippocampal NSCs express apoE, which plays an important role in cell fate determination of NSCs toward neuronal development. ApoE deficiency stimulates astrogenesis and inhibits neurogenesis. ApoE4 decreases hippocampal neurogenesis by inhibiting neuronal maturation of NSCs through impairing GABAergic input onto newborn neurons.