Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2010 Jan;7(1):47-9. doi: 10.1038/nmeth.1404. Epub 2009 Nov 29.

Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.

Author information

  • 1Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.


Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.

[PubMed - indexed for MEDLINE]
Free PMC Article

Images from this publication.See all images (3)Free text

Figure 1
Figure 2
Figure 3
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk