Clin Chem. 1991 Feb;37(2):241-4.

Analysis of apolipoprotein E genotypes by the Amplification Refractory Mutation System.

Source

Department of Clinical Chemistry, Western General Hospital, Edinburgh, U.K.

Abstract

The Amplification Refractory Mutation System (ARMS) has been successfully applied to the detection of apolipoprotein (apo) E genotypes in human DNA extracted from peripheral blood. By using four allele-specific oligonucleotide primers and one common primer, one can identify the three common alleles of the apo E genetic polymorphism, epsilon 2, epsilon 3, and epsilon 4. The system amplifies two sequences of the apo E gene, one of 181 bp and the other 319 bp. These sequences are amplified when DNA containing a particular allele is incubated with its allele-specific oligonucleotide primer and a common primer. The method is simple, reliable, and nonisotopic and obviates the need for digestion with restriction endonucleases or for hybridization with allele-specific oligonucleotide probes. Genotyping DNA by this method overcomes the problem of post-translational modification of the apo E phenotype encountered with isoelectric focusing of the mature plasma apo E protein.

PMID:: 1993332; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Apolipoprotein E phenotypes and genotypes as determined by polymerase chain reaction using allele-specific oligonucleotide probes and the amplification refractory mutation system in children with insulin-dependent diabetes mellitus. [Clin Chim Acta. 1993]
Apolipoprotein E phenotypes and genotypes as determined by polymerase chain reaction using allele-specific oligonucleotide probes and the amplification refractory mutation system in children with insulin-dependent diabetes mellitus.
Stavljenic-Rukavina A, Sertic J, Salzer B, Dumic M, Radica A, Fumic K, Krajina A. Clin Chim Acta. 1993 Jul 16; 216(1-2):191-8.
Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation. [Clin Chem. 1991]
Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation.
Green EK, Bain SC, Day PJ, Barnett AH, Charleson F, Jones AF, Walker MR. Clin Chem. 1991 Jul; 37(7):1263-8.
Common and rare genotypes of human apolipoprotein E determined by specific restriction profiles of polymerase chain reaction-amplified DNA. [Clin Chem. 1994]
Common and rare genotypes of human apolipoprotein E determined by specific restriction profiles of polymerase chain reaction-amplified DNA.
Richard P, Thomas G, de Zulueta MP, De Gennes JL, Thomas M, Cassaigne A, Béréziat G, Iron A. Clin Chem. 1994 Jan; 40(1):24-9.
Review Clinical chemistry of common apolipoprotein E isoforms. [J Chromatogr B Biomed Appl. 1996]
Review Clinical chemistry of common apolipoprotein E isoforms.
Brouwer DA, van Doormaal JJ, Muskiet FA. J Chromatogr B Biomed Appl. 1996 Mar 29; 678(1):23-41.
Review Apolipoprotein E: an important gene and protein to follow in laboratory medicine. [Clin Chem. 1995]
Review Apolipoprotein E: an important gene and protein to follow in laboratory medicine.
Siest G, Pillot T, Régis-Bailly A, Leininger-Muller B, Steinmetz J, Galteau MM, Visvikis S. Clin Chem. 1995 Aug; 41(8 Pt 1):1068-86.

See reviews... See all...

Cited by 6 PubMed Central articles

High serum Abeta and vascular risk factors in first-degree relatives of Alzheimer's disease patients. [Mol Med. 2009]
High serum Abeta and vascular risk factors in first-degree relatives of Alzheimer's disease patients.
Abdullah L, Luis C, Paris D, Ait-ghezala G, Mouzon B, Allen E, Parrish J, Mullan MA, Ferguson S, Wood M, et al. Mol Med. 2009 Mar-Apr; 15(3-4):95-100. Epub 2009 Dec 8.
LNA-enhanced detection of single nucleotide polymorphisms in the apolipoprotein E. [Nucleic Acids Res. 2002]
LNA-enhanced detection of single nucleotide polymorphisms in the apolipoprotein E.
Jacobsen N, Bentzen J, Meldgaard M, Jakobsen MH, Fenger M, Kauppinen S, Skouv J. Nucleic Acids Res. 2002 Oct 1; 30(19):e100.
Estimation of the incidence of a rare genetic disease through a two-tier mutation survey. [Am J Hum Genet. 1993]
Estimation of the incidence of a rare genetic disease through a two-tier mutation survey.
Chakraborty R, Srinivasan MR, Raskin S. Am J Hum Genet. 1993 Jun; 52(6):1129-38.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Analysis of apolipoprotein E genotypes by the Amplification Refractory Mutation ...
Analysis of apolipoprotein E genotypes by the Amplification Refractory Mutation System.
Clin Chem. 1991 Feb ;37(2):241-4.

PubMed
The differential transcriptome and ontology profiles of floating and cumulus gra...
The differential transcriptome and ontology profiles of floating and cumulus granulosa cells in stimulated human antral follicles.
Mol Hum Reprod. 2010 Apr ;16(4):229-40. Epub 2009 Nov 20 .

PubMed
Disease-causing mutations within the lysosomal integral membrane protein type 2 ...
Disease-causing mutations within the lysosomal integral membrane protein type 2 (LIMP-2) reveal the nature of binding to its ligand beta-glucocerebrosidase.
Hum Mol Genet. 2010 Feb 15 ;19(4):563-72. Epub 2009 Nov 20 .

PubMed
Structure of monomeric yeast and mammalian Sec61 complexes interacting with the ...
Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome.
Science. 2009 Dec 4 ;326(5958):1369-73. Epub 2009 Oct 29 .

PubMed
Trisomy 8 as a nonrandom secondary change in myxoid liposarcoma.
Trisomy 8 as a nonrandom secondary change in myxoid liposarcoma.
Cancer Genet Cytogenet. 1991 Feb ;51(2):195-205.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: