Format

Send to:

Choose Destination
See comment in PubMed Commons below
Anal Biochem. 2010 Mar 15;398(2):260-2. doi: 10.1016/j.ab.2009.11.026. Epub 2009 Nov 20.

Comparison of DNA extraction methods for multiplex polymerase chain reaction.

Author information

  • 1Department of Biotechnology, IMCB, University of Tartu, Tartu, Estonia. tviltrop@ut.ee

Abstract

We compared six DNA extraction methods for obtaining DNA from whole blood and saliva for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate saliva sampling as an alternative to blood sampling to obtain DNA for molecular diagnostics, genetic genealogy, and research purposes. The DNA quantity, DNA purity (A(260/280)), PCR inhibition ratio, and mitochondrial DNA/genomic DNA ratio were measured to compare the extraction methods. The different extraction methods resulted in variable DNA quantity and purity, but there were no significant differences in the efficiency of multiplex PCR and oligomicroarray signals after single-base extension on the arrayed primer extension 2 (APEX-2).

Copyright (c) 2009 Elsevier Inc. All rights reserved.

PMID:
19932073
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk