a Camera lucida drawings of BDA-labeled corticospinal tract (CST) fibers that originate in the uninjured hemisphere and project to the side of the spinal cord denervated by injury to the contralateral sensorimoter cortex. Rats were treated with either saline- (left) or inosine (right). b Higher-magnification camera lucida tracing of a fiber in a. Inset shows the presence of synaptic bouton-like structures. c Quantitation of ipsilaterally projecting CST fibers ≥ 40 μm in length in the transverse plane in the denervated dorsal funiculus and gray matter, respectively. Results are normalized by the intensity of staining in the intact CST and are reported as the number of labeled axons per mm length of spinal cord. Inosine does not promote CST axon growth in the absence of brain injury, but amplifies the amount of axon sprouting after a stroke. d Inosine enhances functional recovery. Animals were trained to retrieve food pellets through a restricted opening with either paw prior to surgery and were then tested beginning one week later by a blinded observer. Scores are reported as percentage of pre-operative performance. *, **, ***: Differences significant at P < 0.05, P < 0.01, P < 0.001, respectively. Error bars represent SEM. (from Zai et al., 2009).

(A) Schematic view of motor cortex (red) projections from the brain to the brainstem and cervical spinal cord. In each panel the normal axonal projections from motor cortex are in solid lines. The projections from the cortical region lost from the stroke are in gray dashed lines. The projections that form after stroke are in red dashed lines. In (A), a stroke induces axonal sprouting from contralateral motor cortex into the red nucleus (Papadopoulos et al., 2002) or into the ipsilateral cervical spinal cord (Chen et al., 2002; Zai et al., 2009). These projections form in areas that were denervated by the loss of the stroked cortex. In (B), a stroke induces axonal sprouting from contralateral motor cortex into the ipsilateral striatum and contralateral peri-infarct cortex (Carmichael and Chesselet, 2002). These are areas in which the projections from the stroke site are lost. Stroke also induces several patterns of axonal sprouting in cortex adjacent or ipsilateral to the stroke site, shown in (C). New projections from retrosplenial cortex to peri-infarct cortex form after small strokes in motor cortex (Brown et al., 2009). Somatosensory cortex establishes new direct projections to the ventral premotor cortex. This pattern of axonal sprouting, identified in the squirrel monkey, establishes a long-distance projection from parietal to frontal lobe (Dancause et al., 2005). Premotor cortex in the rate is a site of motor remapping that correlates with functional recovery after cortical injury (Conner et al., '05). Stroke also causes a substantial axonal sprouting response in peri-infarct cortex near the stroke site (Carmichael et al., 2001).