Send to

Choose Destination
See comment in PubMed Commons below
Nat Biotechnol. 2009 Dec;27(12):1173-5. doi: 10.1038/nbt.1589.

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis.

Author information

  • 1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.


We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk