Abstract
The function and metabolic pathway of 3-deoxy-d-manno-octulosonate (KDO) are unclear in plants although it is an essential component in plant cell wall. Here we cloned and characterized a putative Arabidopsis thaliana cytidine monophosphate-KDO synthetase to understand synthetic pathways of KDO. It showed a ubiquitous expression, the activity at an optimal pH of 8.0, and a requirement of Mg2+.
MeSH terms
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Arabidopsis / enzymology*
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Arabidopsis / genetics
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Cell Membrane Permeability / genetics
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Cell Wall / enzymology
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Cloning, Molecular
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Cytidine Monophosphate / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Evolution, Molecular
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Hydrogen-Ion Concentration
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Kinetics
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Ligases / genetics
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Ligases / metabolism
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Nucleotidyltransferases / genetics
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Nucleotidyltransferases / metabolism*
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Polysaccharides, Bacterial / genetics
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Polysaccharides, Bacterial / metabolism
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Structure-Activity Relationship
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Substrate Specificity
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Sugar Acids / chemistry
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Sugar Acids / metabolism
Substances
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Arabidopsis Proteins
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Polysaccharides, Bacterial
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Sugar Acids
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Nucleotidyltransferases
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cytidine monophosphate-3-deoxymannooctulosonate synthetase, Arabidopsis
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Ligases
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Cytidine Monophosphate