Cloning and characterization of cytidine monophosphate-3-deoxy-d-manno-octulosonate synthetase from Arabidopsis thaliana

Ryo Misaki; Hiroyuki Kajiura; Kenji Fujii; Kazuhito Fujiyama; Tatsuji Seki

doi:10.1016/j.jbiosc.2009.05.022

Cloning and characterization of cytidine monophosphate-3-deoxy-d-manno-octulosonate synthetase from Arabidopsis thaliana

J Biosci Bioeng. 2009 Dec;108(6):527-9. doi: 10.1016/j.jbiosc.2009.05.022.

Authors

Ryo Misaki¹, Hiroyuki Kajiura, Kenji Fujii, Kazuhito Fujiyama, Tatsuji Seki

Affiliation

¹ The International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565-0871, Japan.

PMID: 19914588
DOI: 10.1016/j.jbiosc.2009.05.022

Abstract

The function and metabolic pathway of 3-deoxy-d-manno-octulosonate (KDO) are unclear in plants although it is an essential component in plant cell wall. Here we cloned and characterized a putative Arabidopsis thaliana cytidine monophosphate-KDO synthetase to understand synthetic pathways of KDO. It showed a ubiquitous expression, the activity at an optimal pH of 8.0, and a requirement of Mg2+.

MeSH terms

Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Cell Membrane Permeability / genetics
Cell Wall / enzymology
Cloning, Molecular
Cytidine Monophosphate / metabolism
Escherichia coli / genetics
Escherichia coli / metabolism
Evolution, Molecular
Hydrogen-Ion Concentration
Kinetics
Ligases / genetics
Ligases / metabolism
Nucleotidyltransferases / genetics
Nucleotidyltransferases / metabolism*
Polysaccharides, Bacterial / genetics
Polysaccharides, Bacterial / metabolism
Structure-Activity Relationship
Substrate Specificity
Sugar Acids / chemistry
Sugar Acids / metabolism

Substances

Arabidopsis Proteins
Polysaccharides, Bacterial
Sugar Acids
Nucleotidyltransferases
cytidine monophosphate-3-deoxymannooctulosonate synthetase, Arabidopsis
Ligases
Cytidine Monophosphate