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J Am Chem Soc. 2009 Dec 16;131(49):17901-7. doi: 10.1021/ja906979j.

Precise characterization of the conformation fluctuations of freely diffusing DNA: beyond Rouse and Zimm.

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  • 1Edward L. Ginzton Laboratory, Stanford University, Stanford, California 94305, USA. mchalekl@niddk.nih.gov

Abstract

We studied the dynamics of single freely diffusing fluorescence-labeled double-stranded lambda-phage DNA molecules using dual-color 3-dimensional feedback tracking microscopy and intramolecular fluorescence correlation spectroscopy. Our technique is independently sensitive to the molecule's diffusion coefficient D and radius of gyration R(g) and is concentration insensitive, providing greater precision for characterizing the molecule's intramolecular motion than other methods. We measured D = 0.80 +/- 0.05 microm(2)/s and R(g) approximately 420 nm, consistent with the Kirkwood-Riseman prediction for a flexible polymer with strong hydrodynamic interactions (HI), but we find the statistics of intramolecular motion inconsistent with the Zimm model for such a polymer. We address a dispute in the experimental literature, finding that previous measurements on double-stranded DNA likely lacked the sensitivity to distinguish between the Zimm model and the HI-free Rouse model. Finally, we observe fluorescence fluctuations with a correlation time of over 2 s that cannot be explained by either model and propose that they may be signatures of excluded volume interactions.

PMID:
19911791
[PubMed - indexed for MEDLINE]
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