Send to

Choose Destination
See comment in PubMed Commons below
Appl Opt. 2009 Nov 10;48(32):6344-54. doi: 10.1364/AO.48.006344.

Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher.

Author information

  • 1Department of Physics, Creighton University, 2500 California Plaza, Omaha, Nebraska 68178, USA.


The optical stretcher is a dual-beam trap capable of stretching individual cells. Previous studies have used either ray- or wave-optical models to compute the optical pressure on the surface of a spherical cell. We have extended the ray-optics model to account for focusing by the spherical interface and the effects of multiple internal reflections. Simulation results for red-blood cells (RBCs) show that internal reflections can lead to significant perturbation of the deformation, leading to a systematic error in the determination of cellular elasticity. Calibration studies show excellent agreement between the predicted and measured escape force, and RBC stiffness measurements are consistent with literature values. Measurements of the elasticity of murine osteogenic cells reveal that these cells are approximately 5.4 times stiffer than RBCs.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America Icon for PubMed Central
    Loading ...
    Write to the Help Desk