Methods Enzymol. 2009;463:767-85.

Parallel methods for expression and purification.

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Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.

Abstract

Protein properties are highly diverse, making parallel expression and purification a particular challenge. Parallel methods are typically used when a number derivatives of a target protein are desired or when multiple homologs are needed. A typical scenario involves target evaluation, cloning and mutagenesis of the target, expression screening, large-scale expression and purification, and analytical and biophysical testing of the resulting protein. This chapter describes some of the strategies and methods employed for parallel protein expression and purification.

PMID:: 19892201; [PubMed - indexed for MEDLINE]

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Recombinant Proteins

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Parallel methods for expression and purification.

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Abstract

Publication Types, MeSH Terms, Substances

Publication Types

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Supplemental Content

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Cited by 5 PubMed Central articles

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