Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genes Dev. 2009 Nov 1;23(21):2478-83. doi: 10.1101/gad.547309.

PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraurelia.

Author information

  • 1CNRS FRE 3144, Centre de Génétique Moléculaire, Gif-sur-Yvette F-91198 Cedex, France.

Abstract

Programmed genome rearrangements drive functional gene assembly in ciliates during the development of the somatic macronucleus. The elimination of germline sequences is directed by noncoding RNAs and is initiated by DNA double-strand breaks, but the enzymes responsible for DNA cleavage have not been identified. We show here that PiggyMac (Pgm), a domesticated piggyBac transposase, is required for these rearrangements in Paramecium tetraurelia. A GFP-Pgm fusion localizes in developing macronuclei, where rearrangements take place, and RNAi-mediated silencing of PGM abolishes DNA cleavage. This is the first in vivo evidence suggesting an essential endonucleolytic function of a domesticated piggyBac transposase.

PMID:
19884254
[PubMed - indexed for MEDLINE]
PMCID:
PMC2779751
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk