Methods Mol Biol. 2010;576:135-54.

Manual microdissection combined with antisense RNA-longSAGE for the analysis of limited cell numbers.

Source

Institute für Pathology, Saarbrücken Hospital, Saarbrücken, Germany.

Abstract

Establishing a gene expression profile of defined subtypes of cells within an organ is still challenging because it frequently requires microdissection and subsequent amplification of the limited amount of messenger RNA (mRNA) isolated from the microdissected tissue in order to be able to proceed with comprehensive gene expression analyses via microarray or serial analysis of gene expression (SAGE) technology. Here we describe a manual microdissection strategy for the isolation of high-quality RNA. Furthermore, a strategy for combining linear amplification of RNA with longSAGE is described that allows the use of antisense RNA (aRNA) generated via the well-established linear amplification of RNA procedure together with the conventional SAGE or longSAGE technology.

PMID:: 19882261; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Springer

Supplemental Content

Save items

Related citations in PubMed

aRNA-LongSAGE: SAGE with antisense RNA. [Methods Mol Biol. 2008]
aRNA-LongSAGE: SAGE with antisense RNA.
Heidenblut AM. Methods Mol Biol. 2008; 387:39-54.
aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells. [Nucleic Acids Res. 2004]
aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells.
Heidenblut AM, Lüttges J, Buchholz M, Heinitz C, Emmersen J, Nielsen KL, Schreiter P, Souquet M, Nowacki S, Herbrand U, et al. Nucleic Acids Res. 2004 Sep 15; 32(16):e131. Epub 2004 Sep 15.
Optimized procedures for microarray analysis of histological specimens processed by laser capture microdissection. [J Cell Physiol. 2004]
Optimized procedures for microarray analysis of histological specimens processed by laser capture microdissection.
Upson JJ, Stoyanova R, Cooper HS, Patriotis C, Ross EA, Boman B, Clapper ML, Knudson AG, Bellacosa A. J Cell Physiol. 2004 Dec; 201(3):366-73.
Review Expression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis. [Amino Acids. 2005]
Review Expression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis.
McClain KL, Cai YH, Hicks J, Peterson LE, Yan XT, Che S, Ginsberg SD. Amino Acids. 2005 May; 28(3):279-90. Epub 2005 Mar 30.
Review Tissue microdissection techniques in quantitative genome and gene expression analyses. [Histochem Cell Biol. 2001]
Review Tissue microdissection techniques in quantitative genome and gene expression analyses.
Walch A, Specht K, Smida J, Aubele M, Zitzelsberger H, Höfler H, Werner M. Histochem Cell Biol. 2001 Apr; 115(4):269-76.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Manual microdissection combined with antisense RNA-longSAGE for the analysis of ...
Manual microdissection combined with antisense RNA-longSAGE for the analysis of limited cell numbers.
Methods Mol Biol. 2010 ;576:135-54.

PubMed
"Transgenesis, recent technical developments and applications" Nantes, 8th June ...
"Transgenesis, recent technical developments and applications" Nantes, 8th June 2009.
Transgenic Res. 2010 Aug ;19(4):711-4. Epub 2009 Nov 1 .

PubMed
Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25...
Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26.
Theor Appl Genet. 2010 Feb ;120(4):691-7. Epub 2009 Oct 31 .

PubMed
Scleredema Diabeticorum with unusual presentation and fatal outcome.
Scleredema Diabeticorum with unusual presentation and fatal outcome.
Indian J Dermatol. 2008 ;53(4):217-9.

PubMed
Unraveling the mystery of vernix caseosa.
Unraveling the mystery of vernix caseosa.
Indian J Dermatol. 2008 ;53(2):54-60.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Manual microdissection combined with antisense RNA-longSAGE for the analysis of limited cell numbers.

Source

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information