Reprogramming differentially methylated regions (R-DMRs). (a) Enrichment of R-DMRs at CpG island shores. The CHARM array (left, labeled CpG regions) is enriched in CpG islands, and the R-DMRs (right, labeled R-DMR) show marked enrichment at CpG island shores. Islands are denoted as regions that include >50% of a CpG island or are wholly contained in an island, and overlap regions are denoted as regions that include 0.1–50% of a CpG island. Specific base intervals of regions not overlapping islands are indicated; (0–500) means from 1 to 500 bases. Percentage of the distribution (y axis) is given for the CpG regions (CHARM array, null hypothesis) and reprogramming differentially methylated regions (R-DMRs). (b,c) Examples of DMRs. The gene encoding bone morphogenetic protein 7 (BMP7) is indicated in b, and the gene encoding goosecoid (GSC) is indicated in c. In each case, the upper panels show a plot of methylation (M value; see Online Methods) versus genomic location, where the curve represents averaged smoothed M values; the location of CpG dinucleotides (black tick marks), CpG density, location of CpG islands (orange line), as well as the gene annotation are shown. The bottom panels show validation by bisulfite pyrosequencing (mapping to red box in upper panel). Bars represent the mean methylation (triplicate measurement) ± s.d. of iPS cells (pink), fibroblasts (gray) and ES cells (blue) as well as the generally highly methylated HCT116 colon cancer cell line and a generally hypomethylated double DNA methyltransferase 1/3B double knockout line (DKO) derived from it. In each case, five separate CpG sites were assayed quantitatively, shown as differing shades.

DNA methylation at R-DMRs distinguishes normal tissues from each other and colon cancer from normal colon. (a,b) The M values of all tissues from the 4,401 regions (FDR < 0.05) corresponding to R-DMRs (iPS cells compared to parental fibroblasts) were used for unsupervised hierarchical clustering comparing (a) normal brain, spleen and liver (denoted as Br, Sp and Lv, respectively) and (b) colorectal cancer and matched normal colonic mucosa (denoted as T and N, respectively). Notably, all of the normal brain, spleen and liver tissues are completely discriminated by the regions that differ between iPS cells and fibroblasts (R-DMRs). The major branches in the dendrograms correspond perfectly to tissue type. Furthermore, most of the colorectal cancer samples are discriminated from matched normal colonic mucosa by R-DMRs.