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Appl Biochem Biotechnol. 2010 May;161(1-8):93-105. doi: 10.1007/s12010-009-8810-x. Epub 2009 Oct 30.

Ethanol production from sugarcane bagasse by Zymomonas mobilis using simultaneous saccharification and fermentation (SSF) process.

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  • 1Laboratories of Bioprocess Development, Federal University of Rio de Janeiro-Center of Technology-School of Chemistry, 21949-900 Rio de Janeiro, Brazil.


Considerable efforts have been made to utilize agricultural and forest residues as biomass feedstock for the production of second-generation bioethanol as an alternative fuel. Fermentation utilizing strains of Zymomonas mobilis and the use of simultaneous saccharification and fermentation (SSF) process has been proposed. Statistical experimental design was used to optimize the conditions of SSF, evaluating solid content, enzymatic load, and cell concentration. The optimum conditions were found to be solid content (30%), enzymatic load (25 filter paper units/g), and cell concentration (4 g/L), resulting in a maximum ethanol concentration of 60 g/L and a volumetric productivity of 1.5 g L(-1) h(-1).

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