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Am J Trop Med Hyg. 2009 Nov;81(5):917-20. doi: 10.4269/ajtmh.2009.09-0275.

Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction.

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  • 1Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Thailand.


Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard (P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.

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