Send to:

Choose Destination
See comment in PubMed Commons below
Immunol Cell Biol. 2010 Feb;88(2):157-64. doi: 10.1038/icb.2009.80. Epub 2009 Oct 27.

Predicting CD62L expression during the CD8+ T-cell response in vivo.

Author information

  • 1Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Kensington, New South Wales, Australia.


Acute infection leads to CD8(+) T-cell activation, division and differentiation. Following clearance of infection, cells revert to two distinct subsets of memory, central (T(CM)) and effector (T(EM)) memory. Adoptive transfer of naive T-cell receptor transgenic (TCR-tg) T cells has been used to study the differentiation of these memory subsets, which are often discriminated by expression of CD62L. Naive CD8(+) T cells are CD62L(high), and CD62L expression is lost during the 'effector' phase. Adoptive transfer studies show that higher transfer frequencies result in diminished T-cell expansion and a higher proportion CD62L(high). This suggests a relationship between CD62L expression and cell division, where division leads to conversion from CD62L(high) to CD62L(low) phenotype. To address this hypothesis we adoptively transferred graded numbers of OT-1 TCR-tg T cells from naive donors and tracked the kinetics and phenotype of the immune response after infection. We developed a simple mathematical model of division-linked CD62L differentiation, which we compared with the experimental data. Our results show that division-linked differentiation predicts the differences in proportion of cells CD62L(high) observed between responses of different adoptive transfer number and within individual mice. We calculate that approximately 20% of CD62L(high) cells convert to CD62L(low) during each division.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk