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Nucleic Acids Res. 2010 Jan;38(1):72-81. doi: 10.1093/nar/gkp843. Epub 2009 Oct 23.

FtsK translocation on DNA stops at XerCD-dif.

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  • 1Department of Biochemistry, University of Oxford, Oxford, UK.

Abstract

Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes.

PMID:
19854947
[PubMed - indexed for MEDLINE]
PMCID:
PMC2800217
Free PMC Article

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