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Biochim Biophys Acta. 2009 Nov-Dec;1789(11-12):741-50. doi: 10.1016/j.bbagrm.2009.10.002. Epub 2009 Oct 22.

Determination of the core promoter regions of the Saccharomyces cerevisiae RPS3 gene.

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  • 1Labotoray of Biochemistry, School of Life Sciences and Biotechnology, and BioInsititute, Korea University, Seoul 136-701, Korea.

Abstract

Ribosomal protein genes (RPG), which are scattered throughout the genomes of all eukaryotes, are subjected to coordinated expression. In yeast, the expression of RPGs is highly regulated, mainly at the transcriptional level. Recent research has found that many ribosomal proteins (RPs) function in multiple processes in addition to protein synthesis. Therefore, detailed knowledge of promoter architecture as well as gene regulation is important in understanding the multiple cellular processes mediated by RPGs. In this study, we investigated the functional architecture of the yeast RPS3 promoter and identified many putative cis-elements. Using beta-galactosidase reporter analysis and EMSA, the core promoter of RPS3 containing UASrpg and T-rich regions was corroborated. Moreover, the promoter occupancy of RPS3 by three transcription factors was confirmed. Taken together, our results further the current understanding of the promoter architecture and trans-elements of the Saccharomyces cerevisiae RPS3 gene.

PMID:
19853675
[PubMed - indexed for MEDLINE]
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