Appl Environ Microbiol. 2009 Dec;75(24):7870-2. Epub 2009 Oct 16.

Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.

Carroll J, Douarre P, Coffey A, Buckley J, Cashman B, O'Farrell K, O'Mahony J.

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Department of Biological Science, Cork Institute of Technology, Cork, Ireland.

Abstract

A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.

PMID:: 19837835; [PubMed - indexed for MEDLINE]
PMCID:: PMC2794114

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Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.

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