Send to

Choose Destination
See comment in PubMed Commons below
IEEE J Sel Top Quantum Electron. 2008 Jan 1;14(1):4-9.

Multimodal Nonlinear Optical Microscopy and Applications to Central Nervous System Imaging.

Author information

  • 1T. B. Huff is with the Department of Chemistry, Purdue University, West Lafayette, IN 47906 USA ( ).


Multimodal nonlinear optical (NLO) imaging is poised to become a powerful tool in bioimaging given its ability to capitalize on the unique advantages possessed by different NLO imaging modalities. The integration of different imaging modalities such as two-photon-excited fluorescence, sum frequency generation, and coherent anti-Stokes Raman scattering on the same platform can facilitate simultaneous imaging of different biological structures. Parameters to be considered in constructing a multimodal NLO microscope are discussed with emphasis on achieving a compromise in these parameters for efficient signal generation with each imaging modality. As an example of biomedical applications, multimodal NLO imaging is utilized to investigate the central nervous system in healthy and diseased states.

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk