Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis

Stephanie B Schwartz; Stephanie L Mitchell; Kathleen A Thurman; Bernard J Wolff; Jonas M Winchell

doi:10.1128/JCM.01696-09

Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis

J Clin Microbiol. 2009 Dec;47(12):4117-20. doi: 10.1128/JCM.01696-09. Epub 2009 Oct 14.

Authors

Stephanie B Schwartz¹, Stephanie L Mitchell, Kathleen A Thurman, Bernard J Wolff, Jonas M Winchell

Affiliation

¹ Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

Abstract

Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia. Although two genetically distinct types of M. pneumoniae are known, variants of each also exist. We used a real-time PCR high-resolution melt genotyping assay to identify clinical variants which may provide greater insight into the genetic distribution of M. pneumoniae strains.

Publication types

Evaluation Study

MeSH terms

Adhesins, Bacterial / genetics*
Bacterial Typing Techniques / methods
Genetic Variation*
Genotype
Humans
Molecular Sequence Data
Mycoplasma pneumoniae / classification*
Mycoplasma pneumoniae / genetics*
Mycoplasma pneumoniae / isolation & purification
Pneumonia, Mycoplasma / epidemiology
Pneumonia, Mycoplasma / microbiology
Polymerase Chain Reaction / methods*
Sequence Analysis, DNA
Transition Temperature*

Substances

Adhesins, Bacterial
adhesin, Mycoplasma pneumoniae

Associated data

GENBANK/GQ861493
GENBANK/GQ861494
GENBANK/GQ861495