J Biomed Biotechnol. 2009;2009:187426. Epub 2009 Oct 11.

Meso scale discovery and luminex comparative analysis of calbindin D28K.

Sourial S, Marcusson-Ståhl M, Cederbrant K.

Source

Molecular Toxicology, Safety Assessment, AstraZeneca R&D Södertälje, 151 85 Södertälje, Sweden. s.sourial@student.liverpool.ac.uk

Abstract

The sensitivity of different renal regions to xenobiotics requires the development of a multiplex immunoassay for the simultaneous analysis of kidney biomarkers. Calbindin D28K is a distal tubule-specific protein that can be detected in urine under pathological conditions. In this study, a pair of anti-calbindin D28K antibodies was used in an immunoassay for the detection of calbindin D28K expression in rat and human kidney and urine. Comparative analysis of the immunoassay was performed on the Meso Scale Development (MSD) and Luminex platforms. Analysis on both platforms detected calbindin D28K concentrations between 100 ng/mL and 100 pg/mL. Luminex detected 10-fold the amount of calbindin D28K in samples analyzed as compared to MSD, whereas calbindin D28K level in rat and human urine was below detection limit in both platforms. The application of the immunoassays described herein may be useful in toxicological and pathological studies of distal tubular damage in rats and human.

PMID:: 19826628; [PubMed - indexed for MEDLINE]
PMCID: PMC2760132

Free PMC Article

Images from this publication.See all images (1) Free text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Related citations

Review Regulation of renal calbindin-D28K. [Pharmacol Toxicol. 2000]
Review Regulation of renal calbindin-D28K.
Hemmingsen C. Pharmacol Toxicol. 2000; 87 Suppl 3:5-30.
Analysis of rat vitamin D-dependent calbindin-D28k gene expression. [J Biol Chem. 1988]
Analysis of rat vitamin D-dependent calbindin-D28k gene expression.
Varghese S, Lee S, Huang YC, Christakos S. J Biol Chem. 1988 Jul 15; 263(20):9776-84.
Effect of chronic metabolic acidosis on calbindin expression along the rat distal tubule. [J Am Soc Nephrol. 2000]
Effect of chronic metabolic acidosis on calbindin expression along the rat distal tubule.
Rizzo M, Capasso G, Bleich M, Pica A, Grimaldi D, Bindels RJ, Greger R. J Am Soc Nephrol. 2000 Feb; 11(2):203-10.
Estrogen responsiveness of renal calbindin-D28k gene expression in rat kidney. [J Cell Biochem. 1997]
Estrogen responsiveness of renal calbindin-D28k gene expression in rat kidney.
Criddle RA, Zheng MH, Dick IM, Callus B, Prince RL. J Cell Biochem. 1997 Jun 1; 65(3):340-8.
Occurrence and localization of calbindin-D28K in kidney and cerebellum of the slider turtle, Trachemys scripta. [Anat Rec. 1994]
Occurrence and localization of calbindin-D28K in kidney and cerebellum of the slider turtle, Trachemys scripta.
Mutema GK, Rhoten WB. Anat Rec. 1994 Jun; 239(2):185-90.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC

Recent activity

Clear Turn Off Turn On

Meso scale discovery and luminex comparative analysis of calbindin D28K.
Meso scale discovery and luminex comparative analysis of calbindin D28K.
J Biomed Biotechnol. 2009 ;2009:187426. Epub 2009 Oct 11 .

PubMed
Maternal morbidity following a trial of labor after cesarean section vs elective...
Maternal morbidity following a trial of labor after cesarean section vs elective repeat cesarean delivery: a systematic review with metaanalysis.
Am J Obstet Gynecol. 2008 Sep ;199(3):224-31. Epub 2008 Jun 3 .

PubMed
Taxing soft drinks and restricting access to vending machines to curb child obes...
Taxing soft drinks and restricting access to vending machines to curb child obesity.
Health Aff (Millwood). 2010 May ;29(5):1059-66. Epub 2010 Apr 1 .

PubMed
A broader liberty: J.S. Mill, paternalism and the public's health.
A broader liberty: J.S. Mill, paternalism and the public's health.
Public Health. 2009 Mar ;123(3):214-21. Epub 2009 Feb 27 .

PubMed
Mapping cardiac pacemaker circuits: methodological puzzles of the sinoatrial nod...
Mapping cardiac pacemaker circuits: methodological puzzles of the sinoatrial node optical mapping.
Circ Res. 2010 Feb 5 ;106(2):255-71.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: