Correlation between the structural dynamics of the ribosome inferred from multistart simulated annealing (MSSA) refinements and from atomic B factors for (*A–C*) the 50S subunit, (*D–F*) the 30S subunit and (*G* and *H*) RF1 from the RF1–70S termination complex. (*A*, *D*, and *G*) Structures are colored according to RMSD values (pseudo anisotropic B factors) from the refined MSSA model. (*B*, *E*, and *H*) Structures are colored according to anisotropic (*B* and *E*) or isotropic (*H*) atomic B factors from the TLS-refined model. (*C* and *F*) Structures are colored according to solvent accessibility in the ligand-bound 70S termination complex. In all panels, parameters are ramped from blue (lowest values) to red (highest values) using bins containing equal number of atoms. (*I*) Correlation of isotropic atomic B factors with RMS differences between the structures of a MSSA model for the RF1-bound 70S termination complex (black squares) and 23S rRNA (blue triangles) as a function of the number of independently refined structures used for the multiconformer model.

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