Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum

Judith Becker; Corinna Klopprogge; Hartwig Schröder; Christoph Wittmann

doi:10.1128/AEM.01942-09

Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum

Appl Environ Microbiol. 2009 Dec;75(24):7866-9. doi: 10.1128/AEM.01942-09. Epub 2009 Oct 9.

Authors

Judith Becker¹, Corinna Klopprogge, Hartwig Schröder, Christoph Wittmann

Affiliation

¹ Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaussstr. 17, D-38106 Braunschweig, Germany.

Abstract

In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a >40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Citric Acid Cycle*
Corynebacterium glutamicum / genetics*
Corynebacterium glutamicum / metabolism
Gene Expression Profiling
Genetic Engineering*
Lysine / biosynthesis*
Lysine / metabolism

Substances

Bacterial Proteins
Lysine