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J Chromatogr A. 2009 Nov 6;1216(45):7950-6. doi: 10.1016/j.chroma.2009.09.040. Epub 2009 Sep 23.

Binding and elution behavior of proteins on strong cation exchangers.

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  • 1Pfizer Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA.

Abstract

This work provides a broad survey of binding and elution behavior of proteins on strong cation exchangers. Four proteins comprising two monoclonal antibodies, lysozyme, and cytochrome c were used as models in the investigation. Seven chromatography resins with different base matrices were compared. Dynamic binding capacity as a function of salt concentration was examined for a monoclonal antibody and lysozyme. Elution behavior as a function of gradient slope was modeled to determine the characteristic charge, essentially a measure of the number of sites involved in binding, for each protein on each resin. Trends with respect to dynamic binding capacity and elution behavior are analyzed and discussed.

PMID:
19815222
[PubMed - indexed for MEDLINE]
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