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Methods Mol Biol. 2009;557:143-64. doi: 10.1007/978-1-59745-527-5_10.

Genome-wide mapping of meiotic DNA double-strand breaks in Saccharomyces cerevisiae.

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  • 1Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.

Abstract

DNA double-strand breaks (DSBs) initiate meiotic recombination in eukaryotes. We describe two strategies that use microarrays to determine the genome-wide distribution of meiotic DSBs in the yeast Saccharomyces cerevisiae. The first is a chromatin immunoprecipitation (ChIP) approach that targets the Spo11 protein, which remains covalently attached to DSB ends in certain mutant backgrounds. The second approach involves BND cellulose enrichment of the single-strand DNA (ssDNA) recombination intermediate formed by end-resection at DSB sites following Spo11 removal.

PMID:
19799181
[PubMed - indexed for MEDLINE]
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