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Methods Mol Biol. 2009;557:27-33. doi: 10.1007/978-1-59745-527-5_3.

Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae.

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  • Institut Curie, Centre de Recherche, UMR7147-CNRS, Universit√© Pierre et Marie Curie, Paris, France.

Abstract

Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.

PMID:
19799174
[PubMed - indexed for MEDLINE]

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