J Med Microbiol. 2010 Jan;59(Pt 1):65-8.

Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure.

Poppert S, Riecker M, Wellinghausen N, Frickmann H, Essig A.

Source

Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. poppert@bni-hamburg.de

Abstract

This study evaluated fluorescence in situ hybridization (FISH) for rapid identification of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) directly from blood cultures. Initially, 360 blood cultures containing Gram-positive cocci were investigated by a previously described microwave-FISH procedure: 44/49 (89.8 %) S. aureus and 298/299 (99.7 %) CoNS were correctly identified. Because FISH proved useful and reliable but handling was found to be inconvenient, the method was modified by employing a recently developed slide chamber. This reduced the time required from 60 to 30 min. The simplified execution allowed integration of the method into the workflow of a routine laboratory without difficulty. The modified method proved to be highly reliable, identifying 37/37 (100 %) S. aureus and 169/172 (98.2 %) CoNS directly from blood cultures.

PMID:: 19797464; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms

Publication Types

Evaluation Studies

MeSH Terms

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Medical

Staphylococcal Infections - MedlinePlus Health Information

Supplemental Content

Save items

Related citations in PubMed

Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. [Eur J Clin Microbiol Infect Di...]
Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH.
González V, Padilla E, Giménez M, Vilaplana C, Pérez A, Fernández G, Quesada MD, Pallarés MA, Ausina V. Eur J Clin Microbiol Infect Dis. 2004 May; 23(5):396-8. Epub 2004 Apr 27.
Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. [J Clin Microbiol. 2002]
Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.
Oliveira K, Procop GW, Wilson D, Coull J, Stender H. J Clin Microbiol. 2002 Jan; 40(1):247-51.
An evaluation of the advandx Staphylococcus aureus/CNS PNA FISH assay. [Clin Lab Sci. 2009]
An evaluation of the advandx Staphylococcus aureus/CNS PNA FISH assay.
Hensley DM, Tapia R, Encina Y. Clin Lab Sci. 2009 Winter; 22(1):30-3.
Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry. [J Clin Microbiol. 2005]
Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.
Hartmann H, Stender H, Schäfer A, Autenrieth IB, Kempf VA. J Clin Microbiol. 2005 Sep; 43(9):4855-7.
Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. [J Clin Microbiol. 2003]
Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures.
Chapin K, Musgnug M. J Clin Microbiol. 2003 Sep; 41(9):4324-7.

See reviews... See all...

Recent activity

Clear Turn Off Turn On

Accelerated identification of Staphylococcus aureus from blood cultures by a mod...
Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure.
J Med Microbiol. 2010 Jan ;59(Pt 1):65-8.

PubMed
Update on 2009 pandemic influenza A (H1N1) virus.
Update on 2009 pandemic influenza A (H1N1) virus.
Cleve Clin J Med. 2009 Oct ;76(10):577-82.

PubMed
Organization and nucleotide sequence of the 3' end of the human CAD gene.
Organization and nucleotide sequence of the 3' end of the human CAD gene.
DNA Cell Biol. 1990 Nov ;9(9):667-76.

PubMed
W-ChIPMotifs: a web application tool for de novo motif discovery from ChIP-based...
W-ChIPMotifs: a web application tool for de novo motif discovery from ChIP-based high-throughput data.
Bioinformatics. 2009 Dec 1 ;25(23):3191-3. Epub 2009 Oct 1 .

PubMed
Expression of phosphofructokinase in Neisseria meningitidis.
Expression of phosphofructokinase in Neisseria meningitidis.
Microbiology. 2010 Feb ;156(Pt 2):530-42. Epub 2009 Oct 1 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: