J Pharm Pharmacol. 1990 Jun;42(6):450-2.

A rapid gas-liquid chromatographic method for the determination of cotinine and nicotine in biological fluids.

Source

Nicotine Laboratory, New Cross Hospital, London, UK.

Abstract

A rapid method is described for the simultaneous measurement of nicotine and cotinine in biological fluids using capillary column gas-liquid chromatography. Using 100 microliters sample volume the lower limit of detection for both nicotine and cotinine was 100 pg mL-1, allowing the method to be used for the measurement of these compounds in both smokers and non-smokers. The extraction time is 3 min per sample, and by using multi-pipetting and vortexing systems 250 samples can be extracted per day. The average coefficient of variation over the nicotine range 1.0 to 100 ng mL-1 was 3.9% and for cotinine over the range 1.0 to 1000 ng mL-1 was 2.2%. Saliva cotinine concentrations were quantitatively related to passive exposure to parental smoking in a population study of 1118 non-smoking schoolchildren.

PMID:: 1979632; [PubMed - indexed for MEDLINE]

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Determination of cotinine in biological fluids of non-smokers by packed column gas-liquid chromatography. [J Pharm Pharmacol. 1986]
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Feyerabend C, Bryant AE, Jarvis MJ, Russell MA. J Pharm Pharmacol. 1986 Dec; 38(12):917-9.
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Review [Biomarkers of tobacco smoke exposure]. [Ann Biol Clin (Paris). 2002]
Review [Biomarkers of tobacco smoke exposure].
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Review Application of biochemical intake markers to passive smoking measurement and risk estimation.
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Cited by 47 PubMed Central articles

Did smokefree legislation in England reduce exposure to secondhand smoke among nonsmoking adults? Cotinine analysis from the Health Survey for England. [Environ Health Perspect. 2012]
Did smokefree legislation in England reduce exposure to secondhand smoke among nonsmoking adults? Cotinine analysis from the Health Survey for England.
Sims M, Mindell JS, Jarvis MJ, Feyerabend C, Wardle H, Gilmore A. Environ Health Perspect. 2012 Mar; 120(3):425-30. Epub 2011 Dec 5.
Smoking cessation support delivered via mobile phone text messaging (txt2stop): a single-blind, randomised trial. [Lancet. 2011]
Smoking cessation support delivered via mobile phone text messaging (txt2stop): a single-blind, randomised trial.
Free C, Knight R, Robertson S, Whittaker R, Edwards P, Zhou W, Rodgers A, Cairns J, Kenward MG, Roberts I. Lancet. 2011 Jul 2; 378(9785):49-55.
Genetic variation at CHRNA5-CHRNA3-CHRNB4 interacts with smoking status to influence body mass index. [Int J Epidemiol. 2011]
Genetic variation at CHRNA5-CHRNA3-CHRNB4 interacts with smoking status to influence body mass index.
Freathy RM, Kazeem GR, Morris RW, Johnson PC, Paternoster L, Ebrahim S, Hattersley AT, Hill A, Hingorani AD, Holst C, et al. Int J Epidemiol. 2011 Dec; 40(6):1617-28. Epub 2011 May 18.

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