Int J Radiat Biol. 1990 Dec;58(6):989-97.

Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the chromosomal assignment of radiosensitivity gene.

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Radiation Biology Center, Kyoto University, Japan.

Abstract

An immortalized cell line was established from a female ataxia telangiectasia (AT) patient by the transfection of primary skin fibroblasts with origin-defective SV40 DNA. The cell line was characterized by a hypodiploid chromosome constitution and radiation hypersensitivity. The established cell line was used as a recipient for microcell-mediated chromosome transfer. Among seven G418-resistant clones obtained by the fusion with microcells from mouse A9 cells carrying a pSV2neo-tagged normal human chromosome 11, three clones showed restoration of radiation resistance with concomitant gain of an extra intact chromosome 11, while the others contained no recognizable or deleted chromosome 11. The association of the presence of 11q14----qter region with the radioresistance suggests the presence of AT gene in this chromosomal region.

PMID:: 1978855; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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Determination of the chromosomal site for the human radiosensitive ataxia telangiectasia gene by chromosome transfer. [Mutat Res. 1991]
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Cited by 2 PubMed Central articles

Expression cloning of multiple human cDNAs that complement the phenotypic defects of ataxia-telangiectasia group D fibroblasts. [Am J Hum Genet. 1993]
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Meyn MS, Lu-Kuo JM, Herzing LB. Am J Hum Genet. 1993 Dec; 53(6):1206-16.
Ataxia-telangiectasia: linkage evidence for genetic heterogeneity. [Am J Hum Genet. 1992]
Ataxia-telangiectasia: linkage evidence for genetic heterogeneity.
Sobel E, Lange E, Jaspers NG, Chessa L, Sanal O, Shiloh Y, Taylor AM, Weemaes CM, Lange K, Gatti RA. Am J Hum Genet. 1992 Jun; 50(6):1343-8.

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