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J Am Chem Soc. 2009 Sep 30;131(38):13610-2. doi: 10.1021/ja9027023.

Microfluidic device for single-molecule experiments with enhanced photostability.

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  • 1Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Abstract

A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.

PMID:
19772358
[PubMed - indexed for MEDLINE]
PMCID:
PMC2779842
Free PMC Article
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