Cumulated gene counts for MGC, XGC, and ZGC. The progressive addition of clones, measured by genes represented in each collection, is shown for MGC, XGC, and ZGC from the beginning to conclusion of these programs. “Gene Count” is the total final number of RefSeq genes represented by each set of clones. This number includes some noncurated genes (XM accessions) that are not counted in Table 1. “Clone Count” includes all clones, including duplicate transcripts and isoforms. Isoforms constitute 2%–3% of the human, mouse, and rat collections.

MGC progress represented over time by method. (A) Human; (B) mouse. The absolute contribution (by genes represented) of each cloning method is shown for EST-based cloning, PCR-Rescue, and DNA synthesis, over time.

PCR rescue success versus target size. (Black bars) The number of assigned targets in each size range; (white bars) the number of assigned targets that were obtained as full-CDS clones, with the number of clones recovered shown above the bars. The triangles and trendline show the percentage recovered for each size group. Excluded from these calculations are RefSeq targets where the assigned CDS later was changed, suppressed, or withdrawn over the course of the PCR rescue program. Among 8764 human and mouse targets with changed annotation, we obtained a full-CDS clone for 3197 (36%), including one 10.8-kb clone (BC150731).

Synthesis success versus target size. (Black bars) The number of assigned targets in each size range; (white bars) the number of assigned targets that were obtained as full-CDS clones, with the number of clones recovered shown above the bars. The triangles and trendline show the percentage recovered for each size group. RefSeq targets where the assigned CDS later was changed, suppressed, or withdrawn (233 in total) were excluded from these calculations.

Venn diagram comparing the number of loci containing protein-coding genes from MGC, RefSeq, and Ensembl. (A) Human; (B) mouse. The loci were computed by clustering transcripts from all three gene sets based on the overlap of the genomic location of the CDS portion of the exons. When a transcript is not uniquely mapped to the genome, the clusters for all mappings of that transcript were combined and counted as one locus. For human, this resulted in 17,239 loci containing MGC clones, 18,494 loci with RefSeq mRNAs (Pruitt et al. 2009b), and 20,856 Ensembl gene loci (Hubbard et al. 2002). Mouse had 17,455 loci with MGC clones, 19,064 loci with RefSeq mRNAs, and 23,087 Ensembl gene loci. Genes counted as shared between any two gene sets exclude genes in the third set. BLAT (Kent 2002) alignments of MGC clones and RefSeq mRNAs (NM accessions) obtained from the UCSC Genome Browser database (Karolchik et al. 2008) for human genome assembly 36.1 and mouse assembly 37, and Ensembl Release 52 were used in the analysis. Genomic loci serve as an estimate of the number of genes in these data sets. The counts vary from those seen in Table 1, owing to the different method of computation.