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Org Biomol Chem. 2009 Oct 7;7(19):3914-7. doi: 10.1039/b913501d. Epub 2009 Aug 3.

Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases.

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  • 1Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531, Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA.


We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).

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