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Hepatogastroenterology. 2009 Jul-Aug;56(93):1163-8.

A novel method for preparing microbubbles targeting hepatocellular carcinoma.

Author information

  • 1Department of Gastroenterology and Institute of Gastroenterology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, PR China.

Abstract

BACKGROUND/AIMS:

To develop an optimized formula for more stable and effective coupling of albumin microbubbles (MBs) to monoclonal antibodies (mAbs) using a biotin-avidin system.

METHODOLOGY:

Biotin modified albumin MBs were first linked with avidin (Av) followed by treatment with biotinylated mAbs. Each step was monitored using flow cytometry, and the results were summarized with an optimized formula. A hepatocellular carcinoma (HCC) and a normal human hepatocyte cell line were used to examine the effectiveness of this novel linkage strategy.

RESULTS:

The present experiments resulted in 96.05% of MBs bound to their microbubble shells. Av-Bio-MBs were conjugated to mAbs specific for the Hab18G/CD147 cell line. In vitro, CD147-specific MBs were found to bind to HCC cells, but not normal human hepatocyte cells. After exposure to Hab18G rosette-blocking mAb solution, few targeted MBs bound to HCC cells.

CONCLUSIONS:

Conjugation of MBs with biotinylated mAbs forming an Av-Bio-MB system is a novel and attractive tool for HCC-targeting and molecular imaging.

PMID:
19760962
[PubMed - indexed for MEDLINE]
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