Nat Methods. 2009 Oct;6(10):741-4. Epub 2009 Sep 13.

Proteomics strategy for quantitative protein interaction profiling in cell extracts.

Sharma K, Weber C, Bairlein M, Greff Z, Kéri G, Cox J, Olsen JV, Daub H.

Source

Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Abstract

We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands.

PMID:: 19749761; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Related citations

An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells. [Nat Methods. 2006]
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells.
Bürckstümmer T, Bennett KL, Preradovic A, Schütze G, Hantschel O, Superti-Furga G, Bauch A. Nat Methods. 2006 Dec; 3(12):1013-9. Epub 2006 Oct 22.
Identification of uranyl binding proteins from human kidney-2 cell extracts by immobilized uranyl affinity chromatography and mass spectrometry. [J Chromatogr A. 2009]
Identification of uranyl binding proteins from human kidney-2 cell extracts by immobilized uranyl affinity chromatography and mass spectrometry.
Dedieu A, Bérenguer F, Basset C, Prat O, Quéméneur E, Pible O, Vidaud C. J Chromatogr A. 2009 Jul 10; 1216(28):5365-76. Epub 2009 May 18.
Review Approaches for systematic proteome exploration. [Biomol Eng. 2007]
Review Approaches for systematic proteome exploration.
Falk R, Ramström M, Ståhl S, Hober S. Biomol Eng. 2007 Jun; 24(2):155-68. Epub 2007 Feb 6.
Exploring how the organelles are organized. [Nat Methods. 2006]
Exploring how the organelles are organized.
Eisenstein M. Nat Methods. 2006 Jun; 3(6):420-1.
Review Unraveling the dynamics of protein interactions with quantitative mass spectrometry. [Crit Rev Biochem Mol Biol. 2011]
Review Unraveling the dynamics of protein interactions with quantitative mass spectrometry.
Ramisetty SR, Washburn MP. Crit Rev Biochem Mol Biol. 2011 Jun; 46(3):216-28. Epub 2011 Mar 26.

See reviews... See all...

Cited by 4 PubMed Central articles

Disclosure of erlotinib as a multikinase inhibitor in pancreatic ductal adenocarcinoma. [Neoplasia. 2011]
Disclosure of erlotinib as a multikinase inhibitor in pancreatic ductal adenocarcinoma.
Conradt L, Godl K, Schaab C, Tebbe A, Eser S, Diersch S, Michalski CW, Kleeff J, Schnieke A, Schmid RM, et al. Neoplasia. 2011 Nov; 13(11):1026-34.
Review Studying protein-protein affinity and immobilized ligand-protein affinity interactions using MS-based methods. [Anal Bioanal Chem. 2011]
Review Studying protein-protein affinity and immobilized ligand-protein affinity interactions using MS-based methods.
Kool J, Jonker N, Irth H, Niessen WM. Anal Bioanal Chem. 2011 Sep; 401(4):1109-25. Epub 2011 Jul 14.
A computational approach to analyze the mechanism of action of the kinase inhibitor bafetinib. [PLoS Comput Biol. 2010]
A computational approach to analyze the mechanism of action of the kinase inhibitor bafetinib.
Burkard TR, Rix U, Breitwieser FP, Superti-Furga G, Colinge J. PLoS Comput Biol. 2010 Nov 18; 6(11):e1001001. Epub 2010 Nov 18.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Proteomics strategy for quantitative protein interaction profiling in cell extra...
Proteomics strategy for quantitative protein interaction profiling in cell extracts.
Nat Methods. 2009 Oct ;6(10):741-4. Epub 2009 Sep 13 .

PubMed
Intracellular localisation and innate immune responses following Francisella noa...
Intracellular localisation and innate immune responses following Francisella noatunensis infection of Atlantic cod (Gadus morhua) macrophages.
Fish Shellfish Immunol. 2011 Dec ;31(6):993-1004. Epub 2011 Aug 28 .

PubMed
Antioxidant activity of nasunin, an anthocyanin in eggplant peels.
Antioxidant activity of nasunin, an anthocyanin in eggplant peels.
Toxicology. 2000 Aug 7 ;148(2-3):119-23.

PubMed
Risk, Reward, and Economic Decision Making in Aging.
Risk, Reward, and Economic Decision Making in Aging.
J Gerontol B Psychol Sci Soc Sci. 2011 Sep 16. [Epub ahead of print]

PubMed
Cardiomyopathy syndrome of atlantic salmon (Salmo salar L.) is caused by a doubl...
Cardiomyopathy syndrome of atlantic salmon (Salmo salar L.) is caused by a double-stranded RNA virus of the Totiviridae family.
J Virol. 2011 Jun ;85(11):5275-86. Epub 2011 Mar 16 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: