J Immunol Methods. 2009 Oct 31;350(1-2):125-32. Epub 2009 Sep 10.

Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.

Dossus L, Becker S, Achaintre D, Kaaks R, Rinaldi S.

Source

Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, Heidelberg 69120, Germany.

Abstract

Multiplex-based immunoassays (MIA) allow the simultaneous measurement of different cytokines in small sample volumes, but their applicability in samples from "healthy" subjects, as those participating in large-scale epidemiological studies is not well known. We compared measurements of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-1 receptor antagonist (IL-1Ra), C-reactive protein (CRP) and soluble CD40L (sCD40L) obtained by MIA with those obtained by enzyme-linked immunosorbent assays (ELISA) in serum and plasma samples from 36 "healthy" subjects. We observed good correlations between measurements obtained by MIA and ELISA for IL-1Ra, CRP and sCD40L (r>0.80) but poor correlations for IL-6, TNF-alpha and IL-1beta (r<0.40). When comparing MIA plasma and serum measurements, very high correlations were obtained for CRP (r=0.98) and fairly good correlations for IL-1Ra (r=0.60). In conclusion, multiplex-based assays can give an accurate estimate of the relative risk in large-scale epidemiological studies but only for cytokines that are present in relatively large concentrations (ng/mL).

PMID:: 19748508; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Blood Transfusion and Donation - MedlinePlus Health Information

Supplemental Content

Save items

Related citations in PubMed

Increased serum levels of non-collagenous matrix proteins (cartilage oligomeric matrix protein and melanoma inhibitory activity) in marathon runners. [Osteoarthritis Cartilage. 2000]
Increased serum levels of non-collagenous matrix proteins (cartilage oligomeric matrix protein and melanoma inhibitory activity) in marathon runners.
Neidhart M, Müller-Ladner U, Frey W, Bosserhoff AK, Colombani PC, Frey-Rindova P, Hummel KM, Gay RE, Häuselmann H, Gay S. Osteoarthritis Cartilage. 2000 May; 8(3):222-9.
Correlation of XMAP and ELISA cytokine profiles; development and validation for immunotoxicological studies in vitro. [Roum Arch Microbiol Immunol. 2...]
Correlation of XMAP and ELISA cytokine profiles; development and validation for immunotoxicological studies in vitro.
Codorean E, Nichita C, Albulescu L, Răducan E, Popescu ID, Lonită AC, Albulescu R. Roum Arch Microbiol Immunol. 2010 Jan-Mar; 69(1):13-9.
Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models. [J Immunol Methods. 2008]
Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models.
Young SH, Antonini JM, Roberts JR, Erdely AD, Zeidler-Erdely PC. J Immunol Methods. 2008 Feb 29; 331(1-2):59-68. Epub 2007 Dec 17.
Review The immunoassay of cytokines and growth factors in biological fluids. [Dev Biol Stand. 1999]
Review The immunoassay of cytokines and growth factors in biological fluids.
Kopp W, Reynolds C, Ruscetti F. Dev Biol Stand. 1999; 97:29-37.
Review Cytokine quantitation: technologies and applications. [Front Biosci. 2007]
Review Cytokine quantitation: technologies and applications.
Sachdeva N, Asthana D. Front Biosci. 2007 May 1; 12:4682-95. Epub 2007 May 1.

See reviews... See all...

Cited by 5 PubMed Central articles

Temporal reliability of cytokines and growth factors in EDTA plasma. [BMC Res Notes. 2010]
Temporal reliability of cytokines and growth factors in EDTA plasma.
Clendenen TV, Arslan AA, Lokshin AE, Idahl A, Hallmans G, Koenig KL, Marrangoni AM, Nolen BM, Ohlson N, Zeleniuch-Jacquotte A, et al. BMC Res Notes. 2010 Nov 13; 3:302. Epub 2010 Nov 13.
Correlation between serum and plasma antibody titers to mycobacterial antigens. [Clin Vaccine Immunol. 2011]
Correlation between serum and plasma antibody titers to mycobacterial antigens.
Siev M, Yu X, Prados-Rosales R, Martiniuk FT, Casadevall A, Achkar JM. Clin Vaccine Immunol. 2011 Jan; 18(1):173-5. Epub 2010 Nov 3.
Obesity, inflammatory markers, and endometrial cancer risk: a prospective case-control study. [Endocr Relat Cancer. 2010]
Obesity, inflammatory markers, and endometrial cancer risk: a prospective case-control study.
Dossus L, Rinaldi S, Becker S, Lukanova A, Tjonneland A, Olsen A, Stegger J, Overvad K, Chabbert-Buffet N, Jimenez-Corona A, et al. Endocr Relat Cancer. 2010 Dec; 17(4):1007-19. Epub 2010 Oct 29.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Validity of multiplex-based assays for cytokine measurements in serum and plasma...
Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.
J Immunol Methods. 2009 Oct 31 ;350(1-2):125-32. Epub 2009 Sep 10 .

PubMed
A fast and efficient method for quantitative measurement of S-adenosyl-L-methion...
A fast and efficient method for quantitative measurement of S-adenosyl-L-methionine-dependent methyltransferase activity with protein substrates.
Anal Biochem. 2010 Mar 15 ;398(2):218-24. Epub 2009 Sep 11 .

PubMed
Reduction in high blood tumor necrosis factor-alpha levels after manipulative th...
Reduction in high blood tumor necrosis factor-alpha levels after manipulative therapy in 2 cervicogenic headache patients.
J Manipulative Physiol Ther. 2009 Sep ;32(7):586-91.

PubMed
Combined multimodal therapies for chronic tennis elbow: pilot study to test prot...
Combined multimodal therapies for chronic tennis elbow: pilot study to test protocols for a randomized clinical trial.
J Manipulative Physiol Ther. 2009 Sep ;32(7):571-85.

PubMed
Short-term effects of manual therapy on heart rate variability, mood state, and ...
Short-term effects of manual therapy on heart rate variability, mood state, and pressure pain sensitivity in patients with chronic tension-type headache: a pilot study.
J Manipulative Physiol Ther. 2009 Sep ;32(7):527-35.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: