Curr Opin Microbiol. 2009 Oct;12(5):553-8. Epub 2009 Sep 8.

Novel biological insights through metabolomics and 13C-flux analysis.

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Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland.

Abstract

Metabolomics and (13)C-flux analysis have become instrumental for analyzing cellular metabolism and its regulation. Driven primarily by technical advances in mass spectrometry-based analytics, they provide unmatched readouts on metabolic state and activity. Functional genomics leverages metabolomics for the discovery of novel enzymes and unexpected secondary activities of annotated enzymes. (13)C-flux analyses are frequently used for empirical elucidation of pathways in poorly characterized species and for network-wide analysis of mechanisms that realize energy and redox balancing. Integration of metabolomics, (13)C-flux analysis and other data enable the condition-dependent characterization of regulatory circuits that ultimately govern the metabolic phenotype.

PMID:: 19744879; [PubMed - indexed for MEDLINE]

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Review

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Carbon Isotopes

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