Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Curr Opin Chem Biol. 2009 Dec;13(5-6):503-9. doi: 10.1016/j.cbpa.2009.07.026. Epub 2009 Sep 2.

Methods for the proteomic identification of protease substrates.

Author information

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco, UCSF MC 2552, 1700 4th St, San Francisco, CA 94158, United States of America.

Abstract

Proteolysis is a key regulatory post-translational modification in diverse cellular processes including programed cell death, immune function, and development. Tracking proteolytic events has become a focus of researchers assessing the downstream consequences of protease activation. In this review we summarize unbiased methods for identifying protease substrates and tracking the extent of cleavage, a field termed 'degradomics'. These include one-dimensional and two-dimensional gel-based methods for identifying protease substrates, N-terminal peptide identification methods for simultaneously identifying substrates and cleavage sites, and approaches for the quantitation of cleavage events during endogenous proteolysis. Individual methods have identified more than 300 caspase-cleaved targets during apoptosis suggesting broad future applications for these technologies.

PMID:
19729334
[PubMed - indexed for MEDLINE]
PMCID:
PMC2787889
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk