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    Mol Pharm. 2009 Nov-Dec;6(6):1848-55.

    Efficient delivery of antisense oligodeoxyribonucleotide g3139 by human serum albumin-coated liposomes.

    Weecharangsan W, Yu B, Zheng Y, Liu S, Pang JX, Lee LJ, Marcucci G, Lee RJ.

    Source

    Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. wanlop@swu.ac.th

    Abstract

    Human serum albumin (HSA)-coated liposomal formulations were synthesized and evaluated for the delivery of antisense oligodeoxyribonucleotide (ODN) G3139 in KB human oral carcinoma cells. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/alpha-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with G3139 and coated with HSA were investigated for Bcl-2 downregulating activity. Cellular uptake of HSA-coated liposome-ODN complexes was more efficient than the uncoated liposome-ODN complexes. Treatment of the cells with HSA-coated liposome-ODN complexes resulted in efficient Bcl-2 mRNA downregulation that was approximately 3-fold greater than with uncoated liposomes (p < 0.05) and 6-fold greater than with free ODN. The transfection efficiency of liposome-ODN complexes coated with HSA was dependent on the concentration of HSA used and on the contents of alpha-helix and beta-strand in HSA. HSA-coated liposomes are effective delivery vehicles for antisense ODN.

    PMID:
    19725564
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2789904
    Free PMC Article

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