J Proteome Res. 2010 Jan;9(1):95-103.

Application of slicing of one-dimensional gels with subsequent slice-by-slice mass spectrometry for the proteomic profiling of human liver cytochromes P450.

Lisitsa AV, Petushkova NA, Thiele H, Moshkovskii SA, Zgoda VG, Karuzina II, Chernobrovkin AL, Skipenko OG, Archakov AI.

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Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.

Abstract

Sequential thin slicing of one-dimensional electrophoresis gels followed by slice-by-slice mass spectrometry to allow protein identification was used to produce a proteomic map for cytochromes P450. Parallel MALDI-TOF-MS and LC-MS/MS analyses were performed. Combination of the two MS methods increased the quality of protein identification. We have proposed an efficient approach to obtain a comprehensive profile of drug-metabolizing enzymes in the liver that can be used to differentiate between polymorphic variants of cytochromes P450.

PMID:: 19722723; [PubMed - indexed for MEDLINE]

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Application of slicing of one-dimensional gels with subsequent slice-by-slice mass spectrometry for the proteomic profiling of human liver cytochromes P450.

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