Chem Phys Lipids. 1990 Mar;53(2-3):165-75.

Synthesis, spectral properties and enzymatic hydrolysis of fluorescent derivatives of cerebroside sulfate containing long-wavelength-emission probes.

Marchesini S, Preti A, Aleo MF, Casella A, Dagan A, Gatt S.

Source

Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.

Abstract

Fluorescent derivatives of cerebroside sulfate (sulfogalactosyl ceramide, sulfatide) containing long-wavelength-emission fluorophores were synthesized. For this purpose a procedure was developed for preparing a cerebroside 3-sulfate derivative with an amino group on the terminal carbon atom of its fatty acyl residue. The latter compound has been used to prepare cerebroside 3-sulfate, coupled to lissamine-rhodamine, fluoresceine, eosine and NBD. The spectroscopic properties of these compounds, in different solvent systems and when incorporated into micelles of a non-ionic detergent or liposomes of a phospholipid, are reported. Incubation of these respective sulfatides with a human leukocyte preparation, resulted in the formation of the corresponding fluorescent cerebrosides.

PMID:: 1970953; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Elsevier Science

Supplemental Content

Save items

Related citations in PubMed

Synthesis of pyrene derivatives of cerebroside sulfate and their use for determining arylsulfatase A activity. [Biochim Biophys Acta. 1989]
Synthesis of pyrene derivatives of cerebroside sulfate and their use for determining arylsulfatase A activity.
Marchesini S, Viani P, Cestaro B, Gatt S. Biochim Biophys Acta. 1989 Mar 14; 1002(1):14-9.
Improved synthesis of [1-14C]acyl-sphingosine-galactose-3-sulfate (sulfatide) for diagnosis of metachromatic leukodystrophy: usefulness of radioscanning. [Clin Chim Acta. 1991]
Improved synthesis of [1-14C]acyl-sphingosine-galactose-3-sulfate (sulfatide) for diagnosis of metachromatic leukodystrophy: usefulness of radioscanning.
Masson M, Li WX, Fluharty AL, Beaucourt JP, Turpin JC, Baumann N. Clin Chim Acta. 1991 Sep 30; 201(3):157-68.
Correlation of the dispersion state of pyrene cerebroside sulfate and its uptake and degradation by cultured cells. [Biochim Biophys Acta. 1989]
Correlation of the dispersion state of pyrene cerebroside sulfate and its uptake and degradation by cultured cells.
Viani P, Marchesini S, Cestaro B, Gatt S. Biochim Biophys Acta. 1989 Mar 14; 1002(1):20-7.
Assay for cerebroside sulfate (sulfatide) sulfatase in cultured skin fibroblasts with the natural activator protein. [Clin Chim Acta. 1987]
Assay for cerebroside sulfate (sulfatide) sulfatase in cultured skin fibroblasts with the natural activator protein.
Lee-Vaupel M, Conzelmann E. Clin Chim Acta. 1987 Sep 15; 168(1):55-68.
Review The use of coumarins as environmentally-sensitive fluorescent probes of heterogeneous inclusion systems. [Molecules. 2009]
Review The use of coumarins as environmentally-sensitive fluorescent probes of heterogeneous inclusion systems.
Wagner BD. Molecules. 2009 Jan 6; 14(1):210-37. Epub 2009 Jan 6.

See reviews... See all...

Cited by 2 PubMed Central articles

Correction of metachromatic leukodystrophy in the mouse model by transplantation of genetically modified hematopoietic stem cells. [J Clin Invest. 2004]
Correction of metachromatic leukodystrophy in the mouse model by transplantation of genetically modified hematopoietic stem cells.
Biffi A, De Palma M, Quattrini A, Del Carro U, Amadio S, Visigalli I, Sessa M, Fasano S, Brambilla R, Marchesini S, et al. J Clin Invest. 2004 Apr; 113(8):1118-29.
Importance of splicing for prosaposin sorting. [Biochem J. 1999]
Importance of splicing for prosaposin sorting.
Madar-Shapiro L, Pasmanik-Chor M, Vaccaro AM, Dinur T, Dagan A, Gatt S, Horowitz M. Biochem J. 1999 Feb 1; 337 ( Pt 3):433-43.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Synthesis, spectral properties and enzymatic hydrolysis of fluorescent derivativ...
Synthesis, spectral properties and enzymatic hydrolysis of fluorescent derivatives of cerebroside sulfate containing long-wavelength-emission probes.
Chem Phys Lipids. 1990 Mar ;53(2-3):165-75.

PubMed
Short-term prognostic factors in elderly patients seen in emergency departments ...
Short-term prognostic factors in elderly patients seen in emergency departments for acute heart failure.
Rev Esp Cardiol. 2009 Jul ;62(7):757-64.

PubMed
Downregulation of heat shock protein 70 protects rheumatoid arthritis fibroblast...
Downregulation of heat shock protein 70 protects rheumatoid arthritis fibroblast-like synoviocytes from nitric oxide-induced apoptosis.
Arthritis Res Ther. 2009 ;11(4):R130. Epub 2009 Aug 27 .

PubMed
Depletion of T-cell intracellular antigen proteins promotes cell proliferation.
Depletion of T-cell intracellular antigen proteins promotes cell proliferation.
Genome Biol. 2009 ;10(8):R87. Epub 2009 Aug 26 .

PubMed
Altered absorption spectra of iso-1-cytochromes c from mutants of yeast.
Altered absorption spectra of iso-1-cytochromes c from mutants of yeast.
J Biol Chem. 1977 Oct 10 ;252(19):6577-80.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: