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Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20.

Creating bacterial strains from genomes that have been cloned and engineered in yeast.

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  • 1J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.

Abstract

We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.

PMID:
19696314
[PubMed - indexed for MEDLINE]
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