Ultrasound-radiated synthesis of PAMAM-Au nanocomposites and its application on glucose biosensor

Ultrason Sonochem. 2010 Jan;17(1):17-20. doi: 10.1016/j.ultsonch.2009.06.017. Epub 2009 Jul 2.

Abstract

Hybrid nanocomposites of carboxyl-terminated generation 4 (G 4) poly(amidoamine) dendrimers (PAMAM) with gold nanoparticles (AuNPs) encapsulated inside them were synthesized under ultrasound irradiation. The obtained nanocomposites were used to fabricate highly sensitive amperometric glucose biosensor which exhibited a high and reproducible sensitivity of 2.9 mA/mM/cm(2), response time less than 5 s, linear dynamic range from 0.1 to 15.8 microM, correlation coefficient of R(2)=0.9988, and limit of detection (LOD), based on S/N ratio (S/N=3) of 0.05 microM. A value of 2.7 mM for the apparent Michaelis-Menten constant K(M)(app) was obtained. The high sensitivity, wider linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Dendrimers
  • Electrodes*
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / radiation effects
  • Equipment Design
  • Equipment Failure Analysis
  • Glucose / analysis*
  • Glucose / chemistry
  • Glucose Oxidase / chemistry*
  • Glucose Oxidase / ultrastructure
  • Gold / chemistry
  • Gold / radiation effects
  • Materials Testing
  • Nanostructures / chemistry*
  • Nanostructures / radiation effects
  • Nanostructures / ultrastructure
  • Particle Size
  • Polyamines / chemistry*
  • Polyamines / radiation effects
  • Sonication / methods*

Substances

  • Dendrimers
  • Enzymes, Immobilized
  • PAMAM Starburst
  • Polyamines
  • Gold
  • Glucose Oxidase
  • Glucose