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Magn Reson Imaging. 2010 Feb;28(2):153-62. doi: 10.1016/j.mri.2009.07.007. Epub 2009 Aug 19.

Analysis of hyperpolarized dynamic 13C lactate imaging in a transgenic mouse model of prostate cancer.

Author information

  • 1Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA. janinel@radiology.ucsf.edu

Abstract

This study investigated the application of an acquisition that selectively excites the [1-13C]lactate resonance and allows dynamic tracking of the conversion of 13C-lactate from hyperpolarized 13C-pyruvate at a high spatial resolution. In order to characterize metabolic processes occurring in a mouse model of prostate cancer, 20 sequential 3D images of 13C-lactate were acquired 5 s apart using a pulse sequence that incorporated a spectral-spatial excitation pulse and a flyback echo-planar readout to track the time course of newly converted 13C-lactate after injection of prepolarized 13C-pyruvate. The maximum lactate signal (MLS), full-width half-maximum (FWHM), time to the peak 13C-lactate signal (TTP) and area under the dynamic curve were calculated from the dynamic images of 10 TRAMP mice and two wild-type controls. The regional variation in 13C-lactate associated with the injected pyruvate was demonstrated by the peak of the 13C-lactate signal occurring earlier in the kidney than in the tumor region. The intensity of the dynamic 13C-lactate curves also varied spatially within the tumor, illustrating the heterogeneity in metabolism that was most prominent in more advanced stages of disease development. The MLS was significantly higher in TRAMP mice that had advanced disease.

PMID:
19695815
[PubMed - indexed for MEDLINE]
PMCID:
PMC3075841
Free PMC Article

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