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Genome Biol. 2009;10(8):R83. doi: 10.1186/gb-2009-10-8-r83. Epub 2009 Aug 14.

Improved base calling for the Illumina Genome Analyzer using machine learning strategies.

Author information

  • 1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz, 04103 Leipzig, Germany. martin.kircher@eva.mpg.de

Abstract

The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with respect to chemistry and technology than other publicly available packages. Ibis is freely available under the GPL from http://bioinf.eva.mpg.de/Ibis/.

PMID:
19682367
[PubMed - indexed for MEDLINE]
PMCID:
PMC2745764
Free PMC Article

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