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1: Science. 1990 Jan 5;247(4938):83-5.Click here to read Links

Posttranslational glutamylation of alpha-tubulin.

Eddé B, Rossier J, Le Caer JP, Desbruyères E, Gros F, Denoulet P.

Laboratoire de Biochimie Cellulaire, Collège de France, Paris.

The high degree of tubulin heterogeneity in neurons is controlled mainly at the posttranslational level. Several variants of alpha-tubulin can be posttranslationally labeled after incubation of cells with [3H]acetate or [3H]glutamate. Peptides carrying the radioactive moiety were purified by high-performance liquid chromatography. Amino acid analysis, Edman degradation sequencing, and mass spectrometric analysis of these peptides led to the characterization of a posttranslational modification consisting of the successive addition of glutamyl units on the gamma-carboxyl group of a glutamate residue (Glu445). This modification, localized within a region of alpha-tubulin that is important in the interactions of tubulin with microtubule-associated proteins and calcium, could play a role in regulating microtubule dynamics.

PMID: 1967194 [PubMed - indexed for MEDLINE]