Mol Biosyst. 2009 Sep;5(9):931-4. doi: 10.1039/b904228h. Epub 2009 Jul 2.

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.

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The Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA.

Abstract

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

PMID:: 19668857; [PubMed - indexed for MEDLINE]

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Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.
Mol Biosyst. 2009 Sep ;5(9):931-4. doi: 10.1039/b904228h. Epub 2009 Jul 2 .

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Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.

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