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Nat Methods. 2009 Sep;6(9):639-41. doi: 10.1038/nmeth.1361. Epub 2009 Aug 9.

Accurate determination of microbial diversity from 454 pyrosequencing data.

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  • 1Department of Civil Engineering, Rankine Building, University of Glasgow, Glasgow, UK. quince@civil.gla.ac.uk

Abstract

We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

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PMID:
19668203
[PubMed - indexed for MEDLINE]
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